FastEV workflow for optimal biomarker discovery
Extracellular vesicles (EV) and the extended EVome are a potential gold mine of biomarkers. However, the isolation of EVs is typically a slow manual process, various isolation technologies produce heterogeneous outputs – finding a suitable method by testing them one by one requires a lot of resources. FastEV has been developed to make all this much easier.
FastEV can quickly determine the best condition to separate cases from controls or to enrich preknown targets. The optimal FastEV condition can be used easily for thousands of samples allowing high impact biomarker discovery studies.
The FastEV workflow begins with subjecting test samples, e.g. pooled plasmas from cases vs controls, to isolation with an array of FastEV conditions. The obtained isolates undergo content analysis using for example omics or array technologies. The results help to identify optimal FastEV condition, which is applied for high-throughput isolations of samples from large cohorts allowing discovery of biomarkers.